发明名称 Method for forming nerve graft
摘要 A method for forming a nerve graft includes the following steps. A carbon nanotube structure is provided. A hydrophilic layer is formed on a surface of the carbon nanotube structure. The hydrophilic layer is polarized to form a polar surface on the hydrophilic layer. A number of neurons are formed on the polar surface of the hydrophilic layer to form a nerve network. The neurons connect with each other.
申请公布号 US8900866(B2) 申请公布日期 2014.12.02
申请号 US201213349577 申请日期 2012.01.13
申请人 Tsinghua University;Hon Hai Precision Industry Co., Ltd. 发明人 Feng Chen;Fan Li;Zhao Wen-Mei
分类号 C12N5/00;C12N5/02;A61L27/08;A61L27/34 主分类号 C12N5/00
代理机构 Novak Druce Connolly Bove + Quigg LLP 代理人 Novak Druce Connolly Bove + Quigg LLP
主权项 1. A method for forming a nerve graft, comprising: providing a carbon nanotube structure; evaporating or sputtering an inorganic material on a surface of the carbon nanotube structure to form a hydrophilic layer; soaking the hydrophilic layer with a polyamino acid solution or a polyetherimide solution to polarize a surface of the hydrophilic layer to a polar surface, wherein the soaking the hydrophilic layer with the polyamino acid solution or the polyetherimide solution comprises sub-steps of: providing a supporter;placing the carbon nanotube structure having the hydrophilic layer on a surface of the supporter; andsoaking the hydrophilic layer on the carbon nanotube structure on the surface of the supporter with the polyamino acid solution or the polyetherimide solution to polarize a surface of the hydrophilic layer to a polar surface; andcompletely removing the polyamino acid solution or the polyetherimide solution from the polar surface; and seeding and culturing a plurality of neurons on the polar surface until adjacent neurons of the plurality of neurons are connected with each other, and the plurality of neurons form a nerve network.
地址 Beijing CN