| 摘要 |
A method of analyzing a sample includes: providing an element having a base layer; a layer containing streptavidin; a spreading layer, wherein the streptavidin may or may not be in the spreading layer; providing immunocomponents and labeled immunoreactants which may be in the spreading layer or may be combined with the sample; dispensing the sample, and optionally the immunocomponents and a labeled immunoreactant onto the spreading layer at three or more areas, wherein each center of the areas is substantially equidistant to the center formed by the areas, and wherein each area contacts an adjacent area such that wash fluid flow in any direction will contact sample; washing by directing the wash fluid at the center formed by the areas, whereby the wash fluid equally flows over each of the areas; and taking a measurement at each of the areas to determine the presence or concentration of analyte. |
