Process for obtaining a rabbit skin comprising biological active substances

摘要

The present invention relates to a process for obtaining a rabbit skin containing biologically active substances. The rabbit skin extract is obtained by the process including vaccinating a rabbit subcutaneously with vaccinia virus, feeding the rabbit, killing the rabbit when the skin is inflamed, skinning the rabbit within 15 minutes of death, preserving the rabbit skin at −18 degrees Celsius, extracting a portion of the rabbit skin with a phenol solution at 4° C., processing the rabbit skin extract with an acid and an alkali, eluting the rabbit skin extract and fractioning under a nitrogen atmosphere the eluted portion of the rabbit skin extract. The rabbit skin of the present invention can be used for preparing drugs and health foods.

主权项

1. A process for obtaining a rabbit skin extract, said process comprising:
i) inoculating a rabbit subcutaneously with a vaccinia virus, wherein said subcutaneous injection is 0.1-0.4 ml of a vaccinia virus solution containing 106-109 viral particles per one milliliter per each injection site, wherein the total number of injections is from 100 to 250 per rabbit, wherein the rabbit weighs 1.5-3 kg; ii) feeding and monitoring the rabbit for at least 72 hours to monitor development of skin inflammation; iii) sacrificing the rabbit between 72 and 96 hours after inoculation; iv) harvesting the inflamed rabbit skin within 15 minutes of step iii) and preserving the inflamed rabbit skin at 18 degree C; v) obtaining a rabbit skin extract by treating the inflamed rabbit skin obtained in step iv) with a 3% phenol solution at 4 degree C. for at least 72 hours, wherein the ratio between said rabbit skin and said phenol solution is at least 1:4 by weight; vi) processing said rabbit skin extract from step v) to obtain a rabbit skin extract with a pH of 4.5; vii) incubating said processed rabbit skin extract from step vi) with activated charcoal; viii) eluting said processed rabbit skin extract from the activated charcoal from step vii) under a basic pH, thereby obtaining the rabbit skin extract.