| 摘要 |
Disclosed is a method for determining a degree of infection comprising the steps of i) preparing an un-isolated sample by adding a reagent comprising a differentiating marker in an amount sufficient to provide a differentiation between cell types in mammalian milk in a dilution ratio of less than 1:200, preferably less than 1:50 and most preferably less than 1:5; ii) measuring a cell count for each of one or more leukocyte types of somatic cell selected from the group lymphocyte, monocyte, macrophage and polymorphonuclear types of leukocyte differentiated by the marker on the sample by means of a flow cytometer having a detection system sensitive to differences in the differentiating marker resulting from the marker becoming differently associated with different cell types in the sample; and iii) determining an indication of a degree of infection dependent on the measured cell count by employing a comparison of the measured cell count with one or more reference values each being predetermined to be associated with one or more degrees of infection. |
