METHOD OF PRODUCING COPOLYMER OF 3-HYDROXYBUTYRIC ACID AND 3-HYDROXYVALERIC ACID

摘要

The proposed method relates to a microbiological industry and is provided for producing copolymer of 3-hydroxybutyric acid and 3-hydroxyvaleric acid. The proposed method involves culturing a Ralstonia eutropha All-Russian collection of industrial microorganisms V-5786 producer strain in aeration conditions and mixing on a liquid salt medium which contains a hydrogen- and carbon-containing growing substrate which is a gaseous mixture H, CO, CO and Oand is characterized in that culturing a Ralstonia eutropha All-Russian collection of industrial microorganisms V-5786 producer strain is performed in two steps: while continuously feeding a nitrogen source at the first step and without nitrogen and while feeding potassium valerate at the second step. The technical inventive result is aimed at broadening feedstock and reducing costs for producing 3-hydroxybutyric acid and 3-hydroxyvaleric acid copolymer, providing high total copolymer output (at least 80%) due to the optimization of dosing regime in the culture of valeric acid salts.