APPARATUS AND METHOD FOR QUANTITIVE PHASE TOMOGRAPHY THROUGH LINEAR SCANNING WITH COHERENT AND NON-COHERENT DETECTION

摘要

The disclosed invention describes a new apparatus performing a new data acquisition for quantitative refractive index tomography. It is based on a linear scanning of the specimen, opposed to the classical approaches based on rotations of either the sample or the illumination beam, which are based on the illumination with plane waves, which orientation is successively modified in order to acquire angular information. On the contrary, the inventive apparatus and method rely on a specially shaped illumination, which provides straightforwardly an angular distribution in the illumination of the specimen. The specimen can thus be linearly scanned in the object plane in order to acquire the data set enabling tomographic reconstruction, where the different positions directly possess the information on various angles for the incoming wave vectors.

主权项

1. An apparatus for performing quantitative phase tomography on a specimen, comprising:
an illumination source for providing an illuminating beam; an optical device arranged to transform the illuminating beam into a shaped illuminating beam directed according to an optical axis direction to irradiate the specimen; a microscope objective arranged to collect a beam scattered by the specimen; a wave front analyser for wave field amplitude and phase determination and further comprising an array sensor for measuring the intensity of beam scattered by the specimen and collected by the microscope objective, and outputting a measurement signal; displacement means to slide the specimen in a plane normal to the optical axis direction; computing means to process the measurement signal output at the array sensor and delivering quantitative phase tomography images representing the object in 3D and providing quantitative values of the refractive index distribution.