| 摘要 |
PROBLEM TO BE SOLVED: To disclose compositions and methods for partial or complete inactivation of a target gene and also to disclose methods of making and using these compositions (reagents), for example to inactivate a gene in a cell for therapeutic purposes and/or to produce cell lines in which a target gene is inactivated.SOLUTION: For inactivating CCR-5 genes using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain, polynucleotides encoding ZFNs consisting of specific sequences, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs which comprise zinc finger recognition regions of ZFNs are used. |
