System and Method for Producing and Reading DNA Barcodes

摘要

The present disclosure relates to biological material identification systems and methods. DNA oligomers may be used to encode for specific characteristics of biological materials. Encoding may be done by depositing suitable amounts of DNA oligomers onto a portion of a biological material. To identify the biological materials, the encoded portions of the biological materials may be extracted and immersed in buffer solutions. Then, lateral flow tests may be used to decode the DNA for interpretation, creating a readable barcode that may be compared with a database to determine if the biological material may be approved.

主权项

1. A lateral flow test strip comprising:
a sample pad configured to receive a sample solution; a conjugate pad comprising detection conjugates, wherein the detection conjugates are configured to bind to encoding strands included in the sample solution to create a complex of detection conjugates and encoding strands and wherein the detection conjugates carry visible markers; a detection membrane comprising a sample solution and configured to receive the complex and migrate the complex along the detection membrane; test lines comprising immobilized capture strands on a first section of the detection membrane, wherein each capture strand is configured to attach to a predetermined encoding strand present in the sample solution in the detection membrane, and when an encoding strand binds to a capture strand, a visible bar code forms on the surface of the detection membrane as a result of the visible markers carried by the encoding strands; and control lines on a second section of the detection membrane to collect excess complex and indicate if a lateral flow test is complete by binding to excess encoding strands that do not bind to the test lines.