| 摘要 |
A process for producing lipase by microorganisms, preferably on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH 4 ) 2 SO 4 , Na 2 HPO 4 .12H 2 O, MgCl 2 , NH 4 Cl and glucose, using a trace metal solution containing hydrochloric acid, FeSO 4 .7H 2 O, ZnSO 4 .7H 2 O, CaCl 2 .H 2 O, MnSO 4 .5H 2 O, CuSO 4 .5H 2 O, AlCl.6H 2 O, and H 3 BO 3 , and a feeding nutrient solution containing glucose, yeast extract, MgSO 4 .H 2 O, KH 2 PO 4 , Citric Acid, (NH 4 ) 2 SO 4 , Na 2 HPO 4 .12H 2 O, MgCl 2 , and NH 4 Cl, at dissolved oxygen tension of 30-50 %, at temperature of 30-40 °C, while maintaining the pH between 6 and 8, with a glucose content of 15-25 g/L, with isopropylthiogalactoside. |
