IMMUNO-BASED BOTULINUM TOXIN SEROTYPE A ACTIVITY ASSAYS

摘要

The present specification discloses SNAP-25 compositions, methods of making .alpha.-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleav-age product, .alpha.-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleav-age site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutral-izing .alpha.-BoNT/A antibodies.