Method, kit and system for collecting and processing samples for DNA and RNA detection

摘要

This invention relates to a method, kit and system for collecting and processing of samples to release and treat DNA and RNA for gene sequence detection. The method described here in provides for rapid and convenient release, and recovery of DNA and RNA from tissues and cellular materials.

主权项

1. A method for collecting, processing, and analyzing a target nucleic acid from a sample comprising:
(a) providing a sample wherein the sample is a shrimp tissue comprising nucleic acids, wherein the target nucleic acid is diagnostic of a shrimp pathogen-comprising white spot syndrome virus; (b) transferring the sample to a flexible-walled vessel, which can be inverted so that the exterior wall becomes the interior wall and in which sample materials can be agitated and suspended by applying and releasing external pressure to the walls; (c) adding an extraction fluid to the vessel; (d) processing the sample with the extraction fluid and one or more treatment reagents in the vessel to release nucleic acid from the sample to solubilize the nucleic acid and produce a recovery fluid; (e) filtering the recovery fluid produced in step (d) wherein the vessel contains filter media to remove particulates; and (f) transferring a portion of the recovery fluid or product from step (e) to a suitable detection system for detection of the target nucleic acid, wherein the detection system for detection of the target nucleic acid uses PCR or RT-PCR for amplification of the target nucleic acid using diagnostic primer sequences for the shrimp pathogen, and wherein the diagnostic primer sequences for the white spot syndrome virus are selected from: (i) SEQ ID NO: 1 and SEQ ID NO: 2; (ii) SEQ ID NO: 3 and SEQ ID NO: 4; (iii) SEQ ID NO: 5 and SEQ ID NO: 6; and (iv) SEQ ID NO: 7 and SEQ ID NO: 8.