Method for modifying insect resistance of plants by utilizing RNAi technique

摘要

A method for improving insect resistance of plants, in which dsRNA of insect's gene is expressed in plants by using transgenic technique, and then the interfering RNAs are formed in the plants. The interfering RNAs then enter into insects' bodies after being ingested by the insect that eats the plant, and conduct RNA interference against the target gene, thereby expression of the target gene is suppressed by RNA interference. A new plant-mediated method for improving insect resistance by suppressing the growth of insects by RNA interference mechanism.

主权项

1. A method for improving insect resistance of a plant, comprising the following steps:
transfecting a construct for expressing an insect gene dsRNA into a plant cell, a tissue or an organ, wherein said construct for expressing the insect gene dsRNA is double-stranded, and its sense strand or antisense strand contains a structure as shown in Formula 1:
Seqsense-X-Seqantisense  Formula 1wherein,Seqsense is a sense sequence or a fragment of an insect gene, wherein said sense sequence and said fragment are at least 50 bp long;Seqantisense is a sequence or a fragment fully complementary to Seqsense, wherein said sequence and said fragment are at least 50 bp long;X is an intervening sequence between Seqsense and Seqantisense, and said intervening sequence is complementary to neither Seqsense nor Seqantisense,wherein the insect is a Lepidoptera insect, and wherein the insect gene dsRNA can inhibit growth or development of the insect after feeding to the insect, andwherein the insect gene encodes GIP having the amino acid sequence of SEQ ID NO: 2.