| 摘要 |
<p>The invention relates to a process for the fractionation, recovery and purification of CD133+ mononucleated cells from a biological matrix rich in stem cells (umbilical cord, mobilized peripheral blood, bone marrow) using an aqueous two-phase system having at least one step. The invention is suitable for use in the medical field for the recovery of stem cells. The process consists of: construction of the polymer-polymer aqueous two-phase system (in its different modes), addition of the sample, agitation, centrifugation and separation of the phases for the subsequent recovery of same and quantification of CD133+ cells by means of flow cytometry. The phase rich in CD133+ is washed and resuspended in a medium suitable for its subsequent use.</p> |
