HIGH BRIGHTNESS SOLID STATE ILLUMINATION SYSTEM FOR FLUORESCENCE IMAGING AND ANALYSIS

摘要

A solid state illumination system is disclosed, wherein a light source module comprises a first light source, comprising an LED and a phosphor layer, the LED emitting a wavelength λ1 in an absorption band of the phosphor layer for generating longer wavelength broadband light emission from the phosphor, ΔλPHOSPHOR, and a second light source comprising a laser emitting a wavelength λ2 in the absorption band of the phosphor layer. While operating the LED, concurrent laser pumping of the phosphor layer increases the light emission of the phosphor, and provides high brightness emission, e.g. in green, yellow or amber spectral regions. Additional modules, which provide emission at other wavelengths, e.g. in the UV and near UV spectral bands, together with dichroic beam-splitters/combiners allow for an efficient, compact, high-brightness illumination system, suitable for fluorescence imaging and analysis.

主权项

1. A method of providing high brightness illumination for fluorescence imaging and analysis, comprising:
providing a first light source comprising a light emitting device (LED) and a phosphor layer, the LED emitting a first wavelength λ1 within an absorption band of the phosphor layer and the phosphor layer emitting broadband light emission of longer wavelength comprising light in a wavelength band ΔλPHOSPHOR; providing a second light source comprising a laser emitting a second wavelength λ2, within the absorption band of the phosphor layer; and while operating the LED to generate emission at λ1 and ΔλPHOSPHOR, concurrently optically pumping the phosphor layer with laser emission λ2 to increase emission intensity in the phosphor emission wavelength band ΔλPHOSPHOR.