Method and Device for Producing a Bioartificial Tissue Construct

摘要

The tissue construct with viable cells in an extracellular matrix made of fibrin is produced with a special method, in which a matrix material and cells are shaped into a hollow body, in particular a tubular hollow body, by means of a rotational casting method in a hollow mould (1), the method comprising the following steps: (a) introduction of cells of at least one cell type and/or a fibrinogen preparation into the rotating hollow mould (1) with the aid of an applicator (4), said applicator (4) being displaced along the rotational axis during the introduction and step (a) being performed one or more times; (b) continuation of the rotation process until the fibrinogen solidifies into a dimensionally stable matrix, obtaining a primarily solidified tissue construct; (c) removal of the tissue construct from the mould. The construct can also he obtained in a relatively short time from autologous materials.

主权项

1. A method for producing a tissue construct containing viable cells in an extracellular matrix, in which a matrix material and cells are molded by means of a rotary casting method in a hollow mold to give an, in particular, tubular hollow body,
having the following steps: (a) introducing cells of at least one cell type and/or a fibrinogen preparation with the aid of an applicator into the rotating hollow mold, wherein the applicator during the introduction is shifted along the axis of rotation and wherein step (a) is carried out once or several times; (b) continuing the rotary process up to solidification of the fibrinogen to form a dimensionally stable matrix, obtaining a primary-solidified tissue construct; (c) demolding the tissue construct.