ELECTROCHEMICAL ASSAY FOR THE DETECTION OF ENZYMES

摘要

The invention relates to novel compositions and methods for the detection of enzymes using the nuclear reorganization energy, λ, of an electron transfer process.

主权项

1. A method for detecting a target enzyme in a test sample, said method comprising:
(a) adding a test sample comprising a target enzyme to a solid support comprising an electrode comprising:
(i) a self-assembled monolayer (SAM);(ii) a covalently attached eletroactive active moiety (EAM) comprising a transition metal complex with an E0; and(iii) a plurality of substrates attached to said electrode, wherein said substrates are substrates of said enzyme; (b) contacting said target enzyme and said substrates to form a plurality of reactants; and (c) determining the presence of said enzyme by measuring a change of said E0.