Method for determining read error in nucleotide sequence

摘要

A method for determining whether the difference between a first reference sequence and a first sequence of interest which has homology with the first reference sequence comes from a mutation occurring in the first sequence of interest or a read error occurring during sequencing. The method comprises substituting a sequence composed of a predetermined number or more of contiguous nucleotides in each of the first sequence of interest and the first reference sequence by a sequence composed of the predetermined number of contiguous nucleotides to thereby produce a second sequence of interest and a second reference sequence (S103), wherein all of the nucleotides are the same as each other. The method additionally comprises: calculating the edit distance of the second sequence of interest relative to the second reference sequence (S104); and determining whether the difference comes from the abovementioned mutation or the above-mentioned read error on the basis of the edit distance (S105).