RNA-Guided Human Genome Engineering

摘要

A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

主权项

1. A method of integrating foreign DNA into a genomic nucleic acid sequence of a eukaryotic cell comprising
providing to the eukaryotic cell a guide RNA sequence complementary to a target nucleic acid sequence and having a donor sequence hybridized thereto, providing to the eukaryotic cell a Cas9 enzyme that interacts with the guide RNA sequence and cleaves the target nucleic acid sequence in a site specific manner, wherein the guide RNA sequence binds to the complementary target nucleic acid sequence and the Cas9 enzyme cleaves the target nucleic acid sequence in a site specific manner; and wherein the donor sequence is integrated into the genomic nucleic acid sequence.