发明名称 |
METHOD FOR ACQUIRING SAMPLES FOR SPECTRAL BIOCHEMICAL BLOOD ANALYSIS |
摘要 |
FIELD: medicine.SUBSTANCE: method for acquiring samples for the spectral biochemical blood analysis involving preparing and drying blood serum and extracting for the chromatographic examination differs by the fact that a process of a dry matter of blood serum obtaining is performed with stirring constantly at a temperature of 50-60°C for 21-27 hours to produce the dry matter in the form of a plug compacted in the centre and coated with a superficial film; the plug is perforated with a sterile and chemically intact object; 85% methanol is added to a test tube containing the dry matter. The prepared mixture is placed into the stirring device again at a temperature of 48-52°C for 21-27 hours and compacted in a centrifuge at an acceleration of 11500-12500 g. The prepared sample is added to an auto-sampler test tube of a liquid chromatograph in an amount of 3/4 - 2/3 volumes of the test tube.EFFECT: using the present invention enables producing chromatograms with a low-error reproducibility within one sample that is adequate to provide the analysis result reliability with the use of t liquid chromatography. |
申请公布号 |
RU2533258(C1) |
申请公布日期 |
2014.11.20 |
申请号 |
RU20130138665 |
申请日期 |
2013.08.21 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE AVTONOMNOE OBRAZOVATEL'NOE UCHREZHDENIE VYSSHEGO PROFESSIONAL'NOGO OBRAZOVANIJA "SEVERO-VOSTOCHNYJ FEDERAL'NYJ UNIVERSITET IMENI M.K. AMMOSOVA" |
发明人 |
PETROVA PAL'MIRA GEORGIEVNA;KERSHENGOL'TS BORIS MOISEEVICH;NASONOV SERGEJ VIKTOROVICH;KOMZIN KIRILL VASIL'EVICH;TROEV IVAN PETROVICH |
分类号 |
G01N30/02;G01N33/12;G01N33/48 |
主分类号 |
G01N30/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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