CELL KILLING FUSION PEPTIDE EXHIBITING TUMOR CELL-SPECIFIC NECROSIS INDUCTION AND TUMOR REGRESSION

摘要

A cell-killing peptide, more specifically a cell-killing CKP fusion peptide (CTD7:CKP) is disclosed, wherein a cell-killing peptide (CKP) comprising 10 amino acids in MTD of Noxa protein causing cell death, and 7 amino acids targeting a cancer cell are fused. The cell-killing CKP fusion peptide induces strong cell necrosis at various cancer cell lines (HeLa, HCT116, MCF-7, A549, BJAB, CT26, PC3 and the like) and shows strong tumor regression effect at a mouse tumor model using experimental animals, but does not show apoptosis at normal cells. Therefore, it can be broadly used to human body for treating various diseases requiring cell death, particularly, as an anti-cancer drug.

主权项

1. A cancer targeting cell-killing fusion peptide, which comprises the following cancer cell targeting domain (CTD; R1-R7) and cell-killing peptide (CKP), and linker sequence between thereof, and arranged with CTD (R1-R7)-Linker-CKP or CKP-Linker-CTD (R1-R7) configuration:
(a) the cancer cell targeting domain (CTD; R1-R7) consisting of an amino acid sequence expressed as Arg-Xaa-Xaa-Arg-Xaa-Xaa-Arg; (b) the cell-killing peptide (CKP) consisting of the amino acid sequence of SEQ ID No: 21 (KLLNLISKLF) or its homologous sequences having at least 70% of sequence homology; and (c) the linker having an amino acid sequence, wherein n=0-5.