METHOD FOR MEASURING PHYSIOLOGICALLY ACTIVE SUBSTANCE OF BIOLOGICAL ORIGIN

摘要

A method for measuring a specific physiologically active substance of biological origin includes preparing a mixed solution of an AL reagent and a sample containing the physiologically active substance and detecting the aggregation or gelatinization of a protein in the mixed solution while agitating the mixed solution, thereby detecting the physiologically active substance or measuring the concentration of the physiologically active substance in the sample. A predetermined fluorescence emission is imparted to a substance involved in the aggregation or gelatinization in the sample to be measured and/or the AL reagent, and the fluorescence emitted from the substance involved in the aggregation or gelatinization is measured or observed in the mixed solution. In this manner, the aggregation or gelatinization in the mixed solution can be detected.

主权项

1. A method for measuring a physiologically active substance of biological origin by producing a mixed solution of an AL reagent comprising AL which is a hemocyte extract of limulus and a sample comprising a predetermined physiologically active substance of biological origin, and detecting protein aggregation or gelation induced by the reaction between AL and the physiologically active substance in the mixed solution while stirring the mixed solution, whereby detection of the physiologically active substance in the sample or measuring the concentration of the physiologically active substance comprises:
imparting a function of generating a predetermined fluorescence to a substance involved in the aggregation or gelation in the sample and/or the AL reagent, and measuring or observing the fluorescence from the substance involved in the aggregation or gelation in the mixed solution whereby to detection of the aggregation or gelation in the mixed solution results in detection of the physiologically active substance in the sample or measurement of the concentration of the physiologically active substance.