PRODUCTION AND USE OF RAT SPERMATOGONIAL STEM CELL LINES

摘要

A spermatogonial stem cell line that is derived from testes of rats characterized by a desirable genetic background can serve as a source for cells to transplant into male-sterile recipient animals that are immuno-compatible with the spermatogonial line. Rat cells thus transplanted readily develop into fertilization-competent, haploid male gametes, with little or no endogenous sperm competition generated by the testes of the male-sterile recipient. This approach, constituting the first vector system for the use of rat spermatogonial lines from in vitro culture in generating mutant rats on a desired genetic background, effects maximal germline transmission of donor haplotypes from the transplanted spermatogonial cells.

主权项

1. A method for effecting germline transmission of a rat donor haplotype, comprising the steps of (A) providing cells of a spermatogonial stem cell line that is derived from rat testes, said cell line embodying a predetermined genetic background, and then (B) transplanting one or more of said cells into a male-sterile recipient rat that is immunocompatible with said cells, such that transplanted cells develop into fertilization-competent, haploid male gametes.