| 发明名称 |
DETECTION OF ABL MUTANT BY ALLELE-SPECIFIC AMPLIFICATION |
| 摘要 |
The present invention provides a method of detecting for the presence of a mutation in the ABL gene coding for the ABL T315I mutation, the method comprising: (a) providing a sample comprising an mRNA transcript encoding the ABL T315I mutation; (b) performing a reverse-transcription reaction to generate a cDNA sequence from the mRNA transcript; and (c) amplifying a cDNA sequence generated in step (b); wherein the reverse-transcription reaction in step (b) is selective for reverse transcription of the mRNA transcript encoding the ABL T315I mutation over a corresponding alternative ABL transcript that does not contain the mutation. |
| 申请公布号 |
US2014342368(A1) |
申请公布日期 |
2014.11.20 |
| 申请号 |
US201214358176 |
申请日期 |
2012.11.16 |
| 申请人 |
CLARIENT DIAGNOSTIC SERVICES, INC. |
发明人 |
Choppa Paul Christopher |
| 分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of detecting for the presence of a mutation in the ABL gene coding for the ABL T315I mutation, the method comprising:
a) providing a sample comprising an mRNA transcript encoding the ABL T315I mutation; b) performing a reverse-transcription reaction to generate a cDNA sequence from the mRNA transcript; and c) amplifying a cDNA sequence generated in step (b); wherein the reverse-transcription reaction in step (b) is selective for reverse transcription of the mRNA transcript encoding the ABL T315I mutation over a corresponding alternative ABL transcript that does not contain the mutation. |
| 地址 |
ALISO VIEJO CA US |
