Bioimaging nucleic acids, proteins and enzymes

摘要

Systems and methods for labeling, staining, and bioimaging of nucleic acids, their fragments, proteins, polypeptides and enzymes are described. In one aspect, a percentage concentration agarose gel or polyacrylamide gel is generated with agarose or polyacrylamide powder respectively. Silicon nanoparticles are added to the agarose or polyacrylamide gel at a required concentration via the agarose or polyacrylamide powder or after agarose or polyacrylamide solubilization in a loading buffer. The nucleic acid is added to agarose gel slots and proteins or enzymes are added to polyacrylamide gel slots in the loading buffer. The loading buffer is then electrophoresed in each case for an amount of time, causing the added silicon nanoparticles or the added ethidium bromide (after agarose solubilization) to generate a bound and labeled nucleic acid and their fragments and proteins or enzymes (with silicon nanoparticles only).

主权项

1. A method comprising:
bioimaging a nucleic acid or a fragment of the nucleic acid by:
(a) generating a percentage concentration agarose gel with agarose powder;(b) including added silicon nanoparticles at a chosen concentration to the agarose powder or after agarose solubilization in a loading buffer;(c) adding the nucleic acid or the fragment to agarose gel slots in the loading buffer; and(d) electrophoresing the loading buffer for an amount of time, the electrophoresing causing the added silicon nanoparticles to generate a bound, labeled, and stained nucleic acid or fragment by (1) binding the nucleic acid or the fragment, (2) labeling the nucleic acid or the fragment, and (3) staining the nucleic acid or the fragment for bioimaging visualization.