METHOD FOR COMBINED CONDITIONING AND CHEMOSELECTION IN A SINGLE CYCLE
摘要
A method of radiation-free hematopoietic stem cell (HSC) transplantation comprises administering to a mammalian subject one or two doses of 2 to 10 mg/kg body weight of a purine base analog, such as 6TG as a pre-conditioning step. The method further comprises engrafting into the subject hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient donor HSCs within 48 to 72 hours of the pre-conditioning step; and administering to the subject about 1 to 5 mg/kg of the purine base analog every two to four days for two to eight weeks following the engrafting step. The method is performed in the absence of pre-conditioning via radiation. The subject is therefore not treated with myeloablative radiation in preparation for transplantation, and thus the subject is free of myeloablative radiation-induced toxicity.
申请公布号
EP2699247(A4)
申请公布日期
2014.11.19
申请号
EP20120774246
申请日期
2012.04.20
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人
KASAHARA, NORIYUKI;SCHIESTL, ROBERT, H.;HACKE, KATRIN;SZAKMARY, AKOS;CROOKS, GAY, M.