| 摘要 |
<p>In order to provide a method for amplifying a trace amount of mRNA which can amplify a short mRNA and a long mRNA at a same efficiency level, regardless of how long a base sequence is, and to provide a method for use of such a method, a method of the present invention for amplifying a trace amount of mRNA includes the steps of: (i) adding a dummy RNA to a solution containing the trace amount of mRNA, so as to prepare a mixed solution; (ii) synthesizing an anti-sense DNA by reverse transcription, which uses the mixed solution as a template; (iii) synthesizing a sense DNA which is complementary to the anti-sense DNA thus synthesized, so as to generate a double strand DNA made of the sense DNA and the anti-sense DNA; (iv) ligating an RNA polymerase promoter sequence to the double strand DNA thus generated, on a sense DNA 5' end side of the double strand DNA, so as to prepare a double strand DNA for amplification; and (v) amplifying, by using RNA polymerase, an RNA from the double strand DNA for amplification.</p> |
