| 发明名称 |
Method for expansion of human corneal endothelial cells |
| 摘要 |
A method for expanding human corneal endothelial cells includes: (a) providing an amniotic membrane with or without amniotic cells, wherein the amniotic membrane has an extracellular matrix; (b) placing onto the amniotic membrane, a sheet of endothelial layer, or a cell suspension including human corneal endothelial stem cells; and (c) culturing the corneal endothelial cells on the amniotic membrane for a duration sufficient for the corneal endothelial stem cells to expand to an appropriate area. The invention also relates to a method for creating a surgical graft for a recipient site of a patient using the method for expanding human corneal endothelial cells, and the surgical graft prepared therefrom. |
| 申请公布号 |
US8889415(B2) |
申请公布日期 |
2014.11.18 |
| 申请号 |
US200711741903 |
申请日期 |
2007.04.30 |
| 申请人 |
|
发明人 |
Tsai Ray Jui-Fang |
| 分类号 |
C12N5/074;C12N5/0789;C12N5/079;C12N5/0797;C12N5/071;C12N5/00;A61L27/38;A61L27/36;A61K35/12 |
主分类号 |
C12N5/074 |
| 代理机构 |
Wolf, Greenfield & Sacks, P.C. |
代理人 |
Wolf, Greenfield & Sacks, P.C. |
| 主权项 |
1. A method for creating a surgical graft, the method comprising: (a) culturing human corneal endothelial stem cells on a first substrate to allow expansion of the corneal endothelial stem cells, wherein the first substrate is an amniotic membrane having an extracellular matrix; (b) separating the expanded human corneal endothelial stem cells from the amniotic membrane; and (c) placing the cells obtained in (b) on a second substrate to obtain the surgical graft; wherein the second substrate is a corneal disc or a thin layer of an amniotic membrane. |
| 地址 |
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