Chlorella species containing exogenous genes

摘要

The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.

主权项

1. A triglyceride oil produced by cultivating heterotrophically in the dark a genetically engineered Chlorella protothecoides cell comprising an exogenous gene encoding a sucrose invertase, wherein the exogenous gene is codon optimized for expression in the Chlorella protothecoides cell; wherein the cell is cultured on a fixed carbon source selected from the group consisting of glycerol, depolymerized cellulosic material, sucrose, molasses, glucose, galactose, fructose, mannose, acetate, and any combination thereof.