| 发明名称 | Detection methods of NADP(H) using mBFP | ||
| 摘要 | Provided is a detection method of NADP(H) from the change of a fluorescence intensity by a reaction between metagenome-derived blue fluorescent protein (mBFP) and NADPH. More particularly, the present invention relates to methods for detecting NADP(H) using mBFP or his-mBFP, or methods for detecting NADP(H) for measuring an activity of NADP(H) dependent dehydrogenase or oxidoreductase. | ||
| 申请公布号 | US8889345(B2) | 申请公布日期 | 2014.11.18 |
| 申请号 | US201213811809 | 申请日期 | 2012.12.24 |
| 申请人 | Industry Foundation of Chonnam National University | 发明人 | Kim Geun Joong;You Sung Hwan |
| 分类号 | C12Q1/00;G01N33/53;C07K1/00;G01N33/573 | 主分类号 | C12Q1/00 |
| 代理机构 | Sughrue Mion, PLLC | 代理人 | Sughrue Mion, PLLC |
| 主权项 | 1. A detection method of NADP(H) from the change of a fluorescence intensity by a reaction between [i] histidine-tagged metagenome-derived blue fluorescent protein (his-mBFP) having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 3, and [ii] NADPH, wherein said histidine-tagged metagenome-derived blue fluorescent protein is capable of emitting a fluorescence at a wavelength of about 450 nm. | ||
| 地址 | Gwangju KR | ||