发明名称 |
Process for producing protein A-like protein with use of <i>Brevibacillus </i>genus bacterium |
摘要 |
The present invention relates to an efficient and economical process for producing a protein A-like protein. Hosts such as Escherichia coli and Bacillus subtilis have been used in the production of a protein A-like protein using a genetic recombination technique and however, their low productivity has been a big cause of high cost. Thus, it has been desired strongly to immediately establish a technique enabling the inexpensive, large-scale production of a protein A-like protein using recombinant DNA techniques other than Escherichia coli and Bacillus subtilis. The present invention provides a process for producing a protein A-like protein in large amounts, for example, a process comprising allowing a recombinant Brevibacillus genus bacterium to express and secrete the protein in large amounts into a culture solution and separating and collecting the accumulated protein A-like protein from the culture solution. |
申请公布号 |
US8889389(B2) |
申请公布日期 |
2014.11.18 |
申请号 |
US201314066231 |
申请日期 |
2013.10.29 |
申请人 |
Kaneka Corporation |
发明人 |
Kosugi Akihiko;Yajima Kazuyoshi |
分类号 |
C12P21/06;C12N11/14;C12N15/00;C12N1/20;C12P21/02;C07K16/12;C07K14/31 |
主分类号 |
C12P21/06 |
代理机构 |
Sughrue Mion, PLLC |
代理人 |
Sughrue Mion, PLLC |
主权项 |
1. An isolated DNA sequence comprising
a promoter which has a promoter activity when said isolated DNA sequence is transformed into a Brevibacillus genus bacterium, and a DNA sequence encoding a protein A-like protein, wherein said protein A-like protein lacks the cell wall-binding domain X from protein A, and wherein the protein A-like protein is selected from the group consisting of proteins (a) to (c): (a) a protein with immunoglobulin-binding activity, which comprises an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence of either SEQ ID NO: 2 or SEQ ID NO: 4 without cell wall-binding domain X, (b) a protein with immunoglobulin-binding activity, which comprises an arbitrary portion of the amino acid sequence that constitutes the protein (a), and (c) a partial sequence of protein A. |
地址 |
Osaka JP |