发明名称 Phosphine derivatives of fluorescent compounds
摘要 A phosphine derivative of DyLight dyes modified with ethylene glycol or (poly)ethylene glycol groups. In one embodiment, the compounds are useful in chemoselective ligation reactions.
申请公布号 US8889884(B1) 申请公布日期 2014.11.18
申请号 US201213546483 申请日期 2012.07.11
申请人 Pierce Biotechnology, Inc.;Dyomics GmbH 发明人 Hermanson Greg;Czerney Peter T.;Desai Surbhi;Hong Suk J.;Wenzel Matthias S.;Dworecki Boguslawa;Lehmann Frank G.
分类号 C07F9/535 主分类号 C07F9/535
代理机构 Thompson Hine LLP 代理人 Thompson Hine LLP
主权项 1. A compound of general formula IIIwhere each of R1, R2, R5, and R6 is the same or different and is independently selected from the group consisting of an aliphatic, heteroaliphatic, sulfoalkyl group, or heteroaliphatic with terminal SO3; each of R7 and R8 is the same or different and is independently selected from either H, SO3, sulfoalkyl, heteroaliphatic, or heteroaliphatic with terminal SO3; each of R9 and R10 is the same or different and is independently selected from either aryl groups, substituted aryl groups, or cycloalkyl groups; R11 is an electrophilic group selected from the group consisting of a carboxylic acid, an alkyl ester, an aryl ester, a substituted aryl ester, an aldehyde, and amide, an aryl amide, an alkyl halide, a thioester, a sulfonyl ester, and alkyl ketone, an aryl ketone, a substituted aryl ketone, a halosulfonyl, a nitrile, and a nitro; L is a linking group selected from the group consisting of a divalent linear (—(CH2)o—, o=1 to 15), crossed, cyclic, and combinations thereof alkylene group which can be substituted by at least one atom selected from the group consisting of oxygen, substituted nitrogen, and sulfur; Kat is a number of Na+, K+, Ca2+, ammonia, or other cation(s) needed to compensate the negative charge brought by the cyanine; m is an integer from 0 to 5 inclusive; o is an integer from 0 to 12 inclusive; and n is an integer from 1 to 3 inclusive.
地址 Rockford IL US