| 摘要 |
The invention provides for rapid response analysis through lateral flow chromatographic assays of specific antigens present in human or animal fluids, or in agricultural, microbial or biological products, with an audio and visual result of the analysis and when needed, an electronic surge to provide heat for rapid results. A lateral flow device for conducting the analysis includes a plurality of components, and a method for making the device forms components of the device on an elongate, ribbon-like substrate of dielectric material, then folds the substrate into shorter lengths which are then secured together to establish a multiple-layered, self-sustaining structure. |
| 主权项 |
1. A lateral flow device for qualitative or quantitative analysis of a target analyte in a sample, comprising:
a disposable electronic device having a body constructed of juxtaposed lengths of an elongate, folded, ribbon-like substrate of dielectric material secured together to establish a multiple-layered structure, and circuitry formed on the substrate, wherein at least a portion of the circuitry is constructed of a deconstructable material which deconstructs to render the device inoperative in response to reaching a predetermined accumulated amount of time during which the circuitry is operated; wherein the device comprises components disposed on the ribbon-like substrate in the following order: an absorbent sample pad onto which the sample is applied; a conjugate or agent pad comprising at least one specific binding agent specific to the target analyte, and optionally conjugated to a signal-generating agent; a reaction membrane onto which at least one capture agent is immobilized in a line across the membrane as a capture zone or test line, further optionally comprising a control zone, containing a binding agent specific for the capture agent; and a wick or waste reservoir, which draws the sample across the reaction membrane and collects it; and wherein the ribbon-like substrate comprises: at least one circuit to provide an electronic surge to the sample and an electric current source, wherein the electric current source is connected to the circuit for inducing controlled resistive heating therein to heat the sample, wherein the electric current source is a battery; and a means to indicate the results, wherein the means to indicate the result is selected from the group consisting of visual means, audio means, and combinations thereof; and further wherein the speed of the result is increased by application of heat to the sample. |
