NUCLEIC ACID CALIBRATION STANDARDS

摘要

The invention relates to synthetic nucleic acids and in particular, complementary pairs and their use as calibration standards for various techniques used to measure the interaction of biological molecules. The nucleotide sequence of the nucleic acids are non-naturally occurring and the single-stranded nucleic acids are essentially lacking in secondary structure, that is, the stability of hybridization between a single stranded nucleic acid and its complement is greater than any intra-strand pair binding. Characteristics of a binding pair can be used to standardize data across experiments, across instruments and across platforms.