Methods For Culturing Minimally-Passaged Fibroblasts And Uses Thereof

摘要

The present invention relates to novel methods of growing at least 100 million minimally-passaged fibroblasts from a small biopsy specimen. The invention includes methods wherein a small biopsy specimen is needed directly into a large tissue culture flask, and passaged so more than three times.

主权项

1. A method of generating at least 100 million minimally-passaged fibroblasts from a small biopsy specimen, comprising the steps of:
a) digesting the small biopsy specimen with a dissociative or digestive enzyme to form a cell suspension; b) seeding the cell suspension into a first large tissue culture flask; c) culturing a fibroblast cell population in the first large tissue culture flask through at most three passages into sequentially larger tissue culture flasks; and d) harvesting the minimally-passaged fibroblasts.