METHODS OF PRODUCING PROTOPORPHYRIN IX AND BACTERIAL MUTANTS THEREFOR

摘要

The disclosure is directed to a method of producing protoporphyrin IX by (1 ) cultivating a strain of Shewanella bacteria in a culture medium under conditions suitable for growth, and (2) recovering the protoporphyrin IX from the culture medium. The strain of Shewanella bacteria comprises at least one mutant hemH gene which is incapable of normal expression, thereby causing an accumulation of protoporphyrin IX. More specifically, the strain of Shewanella bacteria is a strain of S. loihica, that is S. loihica PV-4. The mutant hemH gene of the strain of Shewanella bacteria may be a mutant of shew_2229 and/or shew_1140.