| 摘要 |
The disclosure provides recombinant viral vectors and attenuated viruses and viral vaccines comprising a virus lacking a functional viral RNA suppressor of host RNAi. The disclosure provides for an attenuated virus that lacks a functional virus-encoded suppressor of RNAi (VSR) of a mammalian subject's RNAi. In a further embodiment, the attenuated virus comprises one or more mutations in the coding sequence for a VSR polypeptide, such as deletions, insertions, substitutions or a combination of any of the foregoing. In another embodiment, the attenuated virus is incapable of inhibiting siRNA biogenesis in a subject. |
