| 摘要 |
<p>The present invention relates to a biodegradable polymannitol-based osmotically gene transporter (PMT) and gene therapy using the same. The biodegradable PMT of the present invention includes an osmotic mannitol skeleton, increases membrane permeability by giving osmotic pressure to a cell membrane and shows drastically enhanced transformation efficiency by promoting intracellular intake course. In addition, the PMT of the present invention shows high combining power with DNA, effectively protects the DNA from nuclease, shows chemicophysical characteristics suitable for using as the gene transporter with increased duration in cytoplasmic, and can be useful as the gene transporter for gene therapy by expressing low cytotoxicity in vitro and in vivo.</p> |
