| 摘要 |
An in vitro method for the propagation of a Strelitzia species from a Strelitzia seed includes chemically scarifying the Strelitzia seed, and effecting surface sterilisation of the scarified seed. The scarified and sterilised seed is inoculated on germination media and allowed to germinate in darkness to establish a germinated seed culture. In a seedling development step, the germinated seed culture is subjected to light conditions for a suitable photoperiod for seedling development, whereafter a shoot explant is obtained by removing the root of the seedling. Apical dominance is uplifted in the shoot explant, and multiple shoot development is initiated on the shoot explant using plant growth regulators. The resulting multiple shoots are separated and axillary shoots subcultured to shoot induction media for further multiplication. Axillary shoots are transferred to root development media for inducing root growth, and hence plantlet regeneration. The resultant in vitro produced plantlets are acclimitised. |
