| 摘要 |
PROBLEM TO BE SOLVED: To provide a chromatography purification method of a non-glycosylated polypeptide recombinantly produced in a prokaryotic cell.SOLUTION: Provided is a purification of a non-glycosylated, heterologous polypeptide wherein the method comprises three chromatography steps of which the first chromatography step selected from i) hydrophobic charge induction chromatography, or ii) hydrophobic interaction chromatography, or iii) affinity chromatography, or iv) ion exchange chromatography, the second chromatography step selected from i) anion exchange chromatography, or ii) cation exchange chromatography, or iii) hydroxylapatite chromatography, or iv) hydrophobic interaction chromatography, or v) hydrophobic charge induction chromatography, and the third chromatography step selected from i) hydrophobic charge induction chromatography, or ii) anion exchange chromatography, or iii) cation exchange chromatography, or iv) hydrophobic interaction chromatography. |
