Site-specific labeling methods and molecules produced thereby

摘要

The present disclosure provides methods of site-specific labeling of antibodies, using proteins having 4'-phosphopantetheinyl transferase activity that catalyze post-translational modification of peptide sequences ("peptide tags") incorporated into one or more specific sites of an antibody of interest. Enzymatic labeling enables quantitative and irreversible covalent modification of a specific serine residue within the peptide tags incorporated into the antibody, and thus creates desirable antibody conjugates.