Viral diagnostics

摘要

The present disclosure provides methods for determining whether a subject is infected with lymphocytic choriomeningitis virus (LCMV). These methods include obtaining a sample from a subject with increased susceptibility to LCMV infection, contacting the sample with one or more compositions for detecting LCMV, and determining whether the one or more compositions for detecting LCMV is associated with a marker of LCMV from the sample, wherein detection of an association indicates that that the subject is infected with LCMV.

主权项

1. A method of assessing lymphocytic choriomeningitis virus (LCMV) infection status or activity in a subject, the method comprising:
selecting a subject for assessment, wherein the subject has been exposed to LCMV and/or is at risk of increased LCMV activity; obtaining a sample from the subject: contacting the sample with a composition for detecting LCMV, wherein the composition comprises at least one isolated monoclonal antibody or antibody fragment thereof, comprising a heavy chain variable region (VH) of monoclonal antibody M87 comprising the amino acid sequence of SEQ ID NO: 74, wherein the VH region comprises a VH CDR1 comprising SEQ ID NO: 76; a VH CDR2 comprising SEQ ID NO: 77; and a VH CDR3 comprising SEQ ID NO: 78, and wherein the antibody or fragment binds specifically to LCMV NP; and determining whether the composition for detecting LCMV is associated with a marker of LCMV from the sample, wherein detection of an association indicates that the subject is infected with and/or has active LCMV.