Human T-cell population

摘要

The present invention has objects to provide a novel human T-cell population having both cytotoxic and immunosuppressive activities, and to a method for preparing the same. The above objects are attained by providing a human T-cell population which is obtainable by coculturing mononuclear cells, collected from human blood, with stroma cells and which has the following features:
(1) being positive for CD3, CD25, CD28 and T-cell antigen receptor αβ;(2) essentially consisting of three groups of a CD4 positive and CD8 positive (CD4+CD8+) T-cell group, a CD4 positive and CD8 dimly positive (CD4+CD8dim) T-cell group, and a CD4 negative and CD8 positive (CD4−CD8+) T-cell group;(3) exerting a cytotoxic activity against the cocultured stroma cells; and(4) exerting an immunosuppressive activity against activated T cells.

主权项

1. A human T-cell population obtainable by coculturing a mononuclear cell collected from human blood with a stroma cell, wherein:
(a) the human blood is selected from the group consisting of cord blood and peripheral blood; (b) the human T-cell population contains cells that express CD3; (c) the human T-cell population contains cells that express CD25; (d) the human T-cell population contains cells that express CD28; (e) the human T-cell population contains cells that express T-cell antigen receptor αβ; (f) the human T-cell population contains subpopulations (i), (ii), and (iii), wherein subpopulation (i) is a CD4 positive and CD8 positive (CD4+CD8+) human T-cell group, subpopulation (ii) is a CD4 positive and CD8 dimly positive (CD4+CD8dim) human T-cell group, and subpopulation (iii) is a CD4 negative and CD8 positive (CD4−CD8+) human T-cell group; (g) wherein at least 90% of the total cells in the human T-cell population are composed of subpopulation (i), subpopulation (ii), and subpopulation (iii); (h) the human T-cell population is free of human or mouse stroma cells; and (i) the human T-cell population produces interleukin-10 (IL-10).