Genetic material manipulation techniques and products thereof

摘要

The presently claimed invention applies to a genetic material processing and manipulation method and related product. The claimed invention relates to a method for changing the inherited characteristics of a cell through micro-beam chromosome modification. In one preferred embodiment, improvements to ‘genomic surgery’ are applied to modify source cell genetic material (101). Source cell (106) is stabilized through micro-pipette (102) applied negative pressure. Excised genetic material (101) undergoes a pre-cutting manipulation step (205) to enhance subsequent genetic manipulation. In an additional embodiment genetic material manipulation is aided by a spindle cutting step (303). The presently claimed invention provides a high quality alternate approach to directed genetic recombination without requiring the use of restriction enzymes and is used for chromosomal repair, removal of defects and new organism creation.

主权项

1. A genetic recombination method, comprising the steps of:
securing source cell with a micropipette exerting negative pressure, micro-beam cutting source genetic material acquiring source genetic material, transporting source genetic material to a target location adjacent to target genetic material, and micro-beam welding said source genetic material to said target genetic material to create combined genetic material.