发明名称 |
Fremgangsmåde til fremstilling af polypeptider i respiratorisk-deficiente celler |
摘要 |
The present invention relates to methods of producing a polypeptide, comprising: (a) introducing into a respiratory-defective mutant of a cell (i) one or more first nucleic acid sequences which complement the respiratory defect and (ii) a second nucleic acid sequence which encodes the polypeptide; (b) cultivating the cell containing the first and second nucleic acid sequences in a culture medium under aerobic conditions suitable for expression of the first and second nucleic acid sequences; and (c) isolating the polypeptide from the cultivation medium of the cell. The present invention also relates to methods for disrupting a gene in a respiratory-deficient mutant cell. The present invention further relates to respiratory-deficient mutant cells and methods for obtaining such mutant cells. |
申请公布号 |
DK1015611(T3) |
申请公布日期 |
2014.11.10 |
申请号 |
DK19980910442T |
申请日期 |
1998.03.17 |
申请人 |
NOVOZYMES A/S;NOVOZYMES, INC. |
发明人 |
JENSEN, EJNER, BECH;CHERRY, JOEL, R.;ELROD, SUSAN, L. |
分类号 |
C12N15/09;C12N15/64;C12N1/14;C12N1/15;C12N1/19;C12N1/21;C12N9/00;C12N9/10;C12N9/88;C12N15/10;C12P21/02;C12R1/69 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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