| 摘要 |
FIELD: chemistry.SUBSTANCE: invention refers to biotechnology and molecular biology. What is presented is the oligonucleotide probe MS8 Flip-R for the fluorescent detection of a histoplasmosis causative agent, Histoplasma capsulatum by a polymerase chain reaction with the nucleotide primers HcMs8s, HcMs8as3 having the following structure: MS8 Flip-R 5'(ROX)-GGCCTGACCAGTATAACCAAGGCC-(BHQ2) 3', wherein ROX is the fluorescent dye carboxy-X-rhodamine, BHQ2 is the fluorescence extinguisher "Black Hole 2".EFFECT: invention can be used in medicine for detecting a genetic material of the histoplasmosis causative agent - H capsulatumin samples for diagnostic purposes in health protection and scientific researches, as enables the high-sensitivity and specificity detecting H capsulatumDNA in pure culture samples and biological material over a short period of time.1 dwg, 1 tbl, 3 ex |
