发明名称 |
RNA INTERFERENCE MEDIATING SMALL RNA MOLECULES |
摘要 |
Abstract Double-stranded RNA (dsRNA) induces sequence-specific post-transcrip 5 tional gene silencing in many organisms by a process known as .RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase IlI like processing reaction from long dsRNA. Chemically synthesized siRNA 10 duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or aptisense target RNA can be cleaved by the produced siRNP complex. |
申请公布号 |
AU2014250627(A1) |
申请公布日期 |
2014.11.06 |
申请号 |
AU20140250627 |
申请日期 |
2014.10.15 |
申请人 |
EUROPAISCHES LABORATORIUM FUR MOLEKULARBIOLOGIE (EMBL);MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V. |
发明人 |
TUSCHL, THOMAS;ELBASHIR, SAYDA MAHGOUB;LENDECKEL, WINFRIED;LUHRMANN, REINHARD;WILM, MATTHIAS |
分类号 |
A01H5/00;A01K67/027;A61K9/00;A61K31/7105;A61K38/00;A61K45/00;A61K48/00;A61P31/12;A61P35/00;A61P37/02;A61P43/00;C07H21/00;C12N1/00;C12N5/10;C12N15/09;C12N15/10;C12N15/113;C12P21/02;C12Q1/02;C12Q1/68;G01N33/15;G01N33/50;G01N33/68 |
主分类号 |
A01H5/00 |
代理机构 |
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代理人 |
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