METHOD FOR DETECTING BIOCHEMICAL OXYGEN DEMAND

摘要

The present invention provides a method for detecting biochemical oxygen demand. Active sludge, surface water, domestic waste water, or industrial waste water comprising microorganism is taken as a water sample with microorganism in the method provided by the present invention. The water sample with microorganism is cultivated to obtain a microorganism film. The blank water sample and the target water sample are made to pass through the microorganism film respectively. The dissolved oxygen reduction current values of the blank water sample and the target water sample are detected. The difference value between the dissolved oxygen reduction current value of the target water sample and that of the blank water sample is obtained. On the basis of the difference value and the predetermined standard curve, the biochemical oxygen demand of the target water sample is acquired. The method provided by the present invention adopts active sludge, surface water, domestic waste water, or industrial waste water comprising microorganism as the water sample with microorganism. The environmental adaptability of the prepared microorganism film is strong, so that one or more of tap water, well water, atmospheric water, or ground water may serve as medium to maintain the physiological activity with no need of providing a buffer solution system.

主权项

1. A method for detecting biochemical oxygen demand (BOD), comprising the steps of:
a) subjecting an air-saturated microorganism-containing water sample to microorganism cultivation to obtain a biofilm; b) allowing an air-saturated blank water sample to pass through the biofilm obtained in said step a), and determining a dissolved oxygen (DO) reduction current of said blank water sample; c) allowing an air-saturated target water sample to pass through the biofilm having been subjected to said step b), and determining a DO reduction current of said target water sample; d) calculating the difference between the DO reduction current of said blank water sample and the DO reduction current of said target water sample according to the DO reduction current of the blank water sample obtained in said step b) and the DO reduction current of the target water sample obtained in said step c); and e) determining BOD of the target water sample according to the difference obtained in said step d) and a predetermined standard curve; wherein said microorganism-containing water sample is activated sludge, surface water, domestic sewage or microorganism-containing industrial wastewater; and said blank water sample is one or more selected from tap water, well water, rain water and groundwater.