METHOD FOR AUTOSOMAL ANALYSING HUMAN SUBJECT OF ANALYTES USING MULTIPLEX GENE AMPLIFICATION

摘要

The present invention relates to a method for analyzing autosomes of a human subject, wherein the method comprises the steps of: (a) obtaining a DNA sample which is an analyzed target; (b) performing multiplex amplification using each of primers which complementarily bonds with amelogenin, D5S818, TH01, D18S51, D3S1358, D7S820, D21S11, D6S1043, human thyroid peroxidase gene (TPOX), von Willebrand factor A (vWA), D16S539, human c-fms proto-oncogene for CSF-1 receptor gene (CSF1PO), D8S1179, D13S317, and human fibrinogen alpha chain (FGA) gene locations of the DNA sample; and (c) determining the allelotype of the gene locations using multiplex amplified products of the step (b) so that the human subject is identified as a gene. According to the present invention, more excellent sensitivity than in existing identifiers and PowerPlex16 is provided, and degeneracy is applied in primer designing, so that amplification can be performed in mutated bonding positions.