ISOTOPIC LABELING OF HIGHER ORGANISMS

摘要

The invention generally relates to metabolic labeling of a target organism with a stable isotope. In a first aspect, the present invention relates to a method for metabolic labeling of a target organism with a stable isotope. The present invention also relates to a method of producing a diet for metabolic labeling of a target organism and to a diet composition for metabolic labeling of a target organism. In a further aspect the invention describes the use of the diet composition according to the present invention for producing a metabolically labeled target organism as a reference for a quantitative proteomic approach. The present invention also relates, in a further aspect, to the use of a diet composition for pulse SILAC labeling. The present invention also relates to the use of a target organism labeled by the method according to the first aspect of the present invention as a spike-in standard in quantitative proteomics approaches.

主权项

1. A method for metabolic labeling of a target organism with a stable isotope, wherein the stable isotope is transferred from a label source to the target organism
a) via one or more food chains, each food chain comprising of one or more cascades of ancestor organisms, wherein each cascade forms the ancestor organism for a successor organism of the following cascade, and wherein at least one food chain comprises i) a straight food chain comprising at least two cascades, wherein at least two ancestor organisms of the at least two cascades are connected in series; or ii) a branched food chain, wherein at least two ancestor organisms of the same or of different cascades are arranged in parallel; or b) via one or more one food chains of a) in combination with at least one synthetic diet labeled with the stable isotope as direct label source; or c) via cells from one or more cell cultures labeled with said stable isotope; or d) via the cells from the one or more cell cultures labeled with the stable isotope in combination with the at least one synthetic diet labeled with the stable isotope as direct label source; or any combination of a) to d).