包括微生物蛋白酶变体的组合物和方法

摘要

本发明提供了枯草蛋白酶变体和包括至少一种此处所述的枯草蛋白酶变体的组合物，以及使用这些变体和组合物的方法。在一些实施方式中，本发明提供了适用于衣物清洁应用的蛋白酶变体。

主权项

一种枯草蛋白酶变体，包括至少一个修饰，其中所述修饰选自：G100C、S101D、S101H、Q103D、M124G、L126G、S132H、S161N、Q275Z和/或选自下列中的至少一组修饰：A001T/L096V/G097S/A098D/D099I，A001V/G097S/A098R/D099G，V004M/A098D/D099G，V004M/D099R/G100S/Z100.01L，V004M/G127C/G128S/P129R，V004M/M119C/D120S，V008I/A098G/D099R，V008I/A098N，V008I/M119C/D120G，Q010L/G097R/A098C/D099S，K012N/A098R/D099V，K012R/G097D/A098H/D099G，A013T/S101R/Q103S，A013V/G097N/A098H/D099G/G160S，A013V/G097S/Z097.01R/A098R，A013V/G100R/S101G，A013V/N118G/M119I，A013V/P129Z/S130H/S132G，P014L/N062D/S101R/Q103S，P014S/A098S/D099G，A015T/V068N/A069G，L016M/G128R/P129R/Z129.01G，H017Q/G127R/P129G，H017Y/A098R/D099I，H017Y/A098R/D099Z/S101G，H017Y/P129R，H017Y/S130Y/G131S/S132L，Q019H/G128R/Z128.01R/P129D，Q019H/Z128.01G/Z128.02G，G020D/G097R/A098G/D099C，G020D/P129Z/S130G/G131R/S132C，N025Y/S101L/Q103N，A029V/G097R/A098S，S033I/T066G/H067R/V068D/A216E，S038F/A098R/D099S，V044I/G097C/A098D/D099G，V044I/G097R/A098R，V044I/G100S/S101G，A045V/G097C/A098R/D099S，A048V/G097L/A098G/D099S，A048V/G100R/S101G，A048V/G128S/P129L/Z129.01G，M050I/A098G，S053F/S130I/S132G，S053T/D099H/S101Z，E054D/A098R/D099G，T055I/S101R/Q103R，T055S/T066I/Z066.01G/Z066.02G，Q059H/D099S/S101G，Q059H/M119S/D120R，Q059K/M119V/V121C，Q059L/P129G，N061D/G128R/P129V/Z129.01G，S063T/G097D/A098R/D099S，G065R/Z101.01R/Z101.02G，T066S/H067S，V068C/A069G，V068C/A069G/H238Q，V068G/A069G，V068G/A069G/V150I，V068G/A069S，V068I/A069G/A144V，V068I/G097D/A098G/D099Z/G100R，V068L/A069G，V068L/A069S，V068S/A069G，V068S/A069G/A116T，A069G/T071G，A069G/T071I，L075G/N076G/N077Z，L075R/N076G/N077Z，N076D/D099R/Z100.01G，I079F/A098G，V081A/S101R/Q103V，V081I/G097R/A098G/D099C，V084D/G097S/A098G/D099N，V084I/G127C/P129G，V084I/G127L，A085V/A098G，A085V/G097R/A098S/D099N，A085V/G128S/P129R/Z129.01G，A085V/N218I，A085V/S101G，A085V/S204P，A085V/Z099.01H/S101R，P086S/G131D/S132G，P086T/S101D/Q103S/V147I，A088S/S101R/Q103V，A088T/A098R/D099R，A088T/D099G/Z100.01G，A088T/G127N/P129R，A088T/G127S/P129R/Q206R/A273S，A088T/P129S/G146D，A088T/S101G，A088T/S130R/G131H/S132N，A088V/G100Z/S101C/Q103R，A088V/P129Z，A092G/S249R，A092G/V093C，A092G/V093I，A092G/V093L，A092L/V093G/K094Z，A092S/K094V，A092S/V093C，A092S/V093G，A092S/V093S，A092V/V093L，V093I/G128D/P129R，V093I/P129Z，V093R/Z093.01G/K094R，K094E/V095D，K094S/V148I，L096I/N118R/M119C/D120G/A151V，Z096.01D/A098R，Z096.01H/A098G，Z096.01N/A098H，Z096.01R/A098C，Z096.01R/A098G，Z096.01R/A098G/V192I，Z096.01R/A098R，Z096.01R/A098R/V192A，Z096.01R/A098S，Z096.01R/A098S/G157S，Z096.01R/Z096.02G，Z096.01R/Z096.02G/V147I，Z096.01S/A098G，Z096.01S/A098N，G097C/A098D/D099S，G097C/A098G/D099G，G097C/A098G/D099N，G097C/A098G/D099S，G097C/A098G/D099S/G100Z，G097C/A098G/D099V，G097C/A098N/D099S，G097C/A098R，G097C/A098R/D099G，G097C/A098R/D099H，G097C/A098R/D099H，G097C/A098R/D099N，G097C/A098R/D099R，G097C/A098R/D099R/N109D/A114S，G097C/A098S/D099H/A151V，G097C/A098V/D099S，G097C/Z097.01G/A098H/T253I，G097C/Z097.01S/A098G，G097D/A098C，G097D/A098G/D099G，G097D/A098G/D099R，G097D/A098H/D099R，G097D/A098H/D099V，G097D/A098R/D099G，G097D/A098R/D099R，G097D/A098R/D099S，G097D/A098R/D099Z，G097D/G100C/S101R，G097D/G100R/S101G，G097H/A098C/D099G，G097H/A098D/D099N，G097H/A098G，G097H/A098G/D099G，G097H/A098G/D099R，G097H/A098G/D099Z，G097H/A098G/D099Z/A151V，G097H/A098G/D099Z/G100R，G097H/A098G/D099Z/G100V，G097H/A098G/D099Z/S101N/G166S，G097H/A098R，G097H/A098R/D099G，G097H/A098R/D099L，G097H/A098R/D099N，G097H/A098S/D099G，G097H/A098S/D099I，G097H/A098S/D099L，G097H/A098S/D099S/K237E，G097L/A098C/D099S，G097L/A098G/D099S，G097L/A098G/D099S/G100Z，G097L/A098G/D099Z，G097L/A098H，G097L/A098R/D099N，G097L/A098V，G097N/A098G/D099C，G097N/A098G/D099R，G097N/A098G/D099R/V270I，G097N/A098G/D099S，G097N/A098G/D099V，G097N/A098G/D099V/A151V，G097N/A098G/D099V/S101F，G097N/A098H/D099N，G097N/A098R/D099R，G097N/A098R/D099S，G097N/A098R/D099V，G097N/A098S/D099S，G097R/A098C，G097R/A098C/D099S，G097R/A098F/A200V，G097R/A098G，G097R/A098G/D099C，G097R/A098G/D099G，G097R/A098G/D099G/A273T，G097R/A098G/D099S，G097R/A098G/D099S/A133D，G097R/A098G/D099V，G097R/A098H/D099C/S182N，G097R/A098H/D099G，G097R/A098N/D099G，G097R/A098N/D099Z，G097R/A098R，G097R/A098R/D099C，G097R/A098R/D099G，G097R/A098R/D099S，G097R/A098S/D099C，G097R/A098S/D099G，G097R/A098S/D099L，G097R/A098S/D099R，G097R/A098S/D099S，G097R/A098S/D099V，G097R/A098V/D099G，G097R/Z097.01H/A098C，G097R/Z097.01R/A098G，G097R/Z097.01R/A098G/V147I，G097R/Z097.01R/A098S，G097R/Z097.01R，G097R/Z097.01S/A098G，G097R/Z097.01S/A098R，G097S/A098C，G097S/A098C/D099G，G097S/A098C/D099H，G097S/A098C/D099L，G097S/A098C/D099R，G097S/A098C/D099S，G097S/A098C/D099Z，G097S/A098G/D099F，G097S/A098G/D099G，G097S/A098G/D099G，G097S/A098G/D099S，G097S/A098G/D099Z，G097S/A098G/D099Z/G100R，G097S/A098H/D099C，G097S/A098H/D099G，G097S/A098N/D099G，G097S/A098R，G097S/A098R/D099C，G097S/A098R/D099G，G097S/A098R/D099L，G097S/A098R/D099V，G097S/A098R/D099Y，G097S/A098S/D099G，G097S/A098S/D099G/A133V/A153V/S236Y，G097S/A098S/D099G/A153V，G097S/A098S/D099Z，G097S/Z097.01D/A098G/A144T/Q271H，G097S/Z097.01G/A098V，G097S/Z097.01S/A098G，G097S/Z097.01S/A098G/A273T，G097S/Z097.01Y，G097V/A098R，G097V/A098R/D099G，G097V/A098S/D099S，G097Y/A098H/D099N，G097Z/A098S/D099H，Z097.01D/A098R/D099G，Z097.01D/A098S/D099L，Z097.01D/A098V/D099G，Z097.01G，Z097.01G/A098G，Z097.01G/A098G/D099S，Z097.01G/A098R，Z097.01G/A098R/D099C，Z097.01G/A098R/D099G，Z097.01G/A098R/D099S，A098C/D099G，A098C/D099G/A116T，A098C/D099H，A098C/D099L，A098C/D099L/K213R，A098C/D099L，A098C/D099N，A098C/D099R，A098C/D099S，A098C/D099S/P194L，A098D/D099C，A098D/D099G，A098D/D099G/G100L，A098D/D099H/G100S，A098D/D099H/H238Y，A098D/D099R，A098D/D099R/G100D，A098D/D099R/G160S，A098F/D099S/G100L，A098G/D099CA098G/D099G，A098G/D099G/A228T，A098G/D099G/G100H，A098G/D099G/N243D，A098G/D099G/S101P，A098G/D099G/T244S，A098G/D099G，A098G/D099H，A098G/D099L，A098G/D099R，A098G/D099R/A116T，A098G/D099R/G100D，A098G/D099R/V148I，A098G/D099S，A098G/D099S/L267M，A098G/D099V，A098G/D099Z，A098H/D099C，A098H/D099G，A098H/D099G/A216T，A098H/D099G/G100D，A098H/D099G/G100N/L267M，A098H/D099G/G100S，A098H/D099G/V143I，A098H/D099R，A098H/D099R/G100S，A098H/D099S/G100C，A098H/D099S/G100N，A098H/D099Y，A098I/D099G，A098I/D099S，A098L/D099G，A098L/D099S，A098N/D099G，A098N/D099L，A098N/D099S，A098R/A144T，A098R/A274V，A098R/D099C，A098R/D099G，A098R/D099G/G100C，A098R/D099G/G100D，A098R/D099G/G100L/S145T，A098R/D099G/G100N，A098R/D099G/G100R，A098R/D099G/G100S，A098R/D099G/V150I，A098R/D099H，A098R/D099I，A098R/D099L，A098R/D099N，A098R/D099N/G100L，A098R/D099R，A098R/D099R/G100D，A098R/D099R/S101Z，A098R/D099R/S130F，A098R/D099R，A098R/D099S，A098R/D099S/G100L/A153V，A098R/D099S，A098R/D099V，A098R/D099Z/S101G，A098R/G100C/A137T，A098R/G100S，A098S/D099C，A098S/D099G，A098S/D099G/G100C，A098S/D099G/G100D，A098S/D099G/G100R/L250I，A098S/D099G/G100S，A098S/D099G/Z099.01R，A098S/D099H，A098S/D099H/M124V，A098S/D099H/Z099.01R/G169A/V180A，A098S/D099R，A098S/D099R/G100L，A098S/D099R/G154S，A098S/D099R/G166S，A098S/D099R/Z100.01G，A098S/D099S，A098S/D099S/Z099.01R，A098S/D099V，A098T/P129Z/S130R/S132G，A098T/S101G，A098V/D099G，A098V/D099G/G100D，A098V/D099G/G100D/A153V，A098V/D099G/G100S，A098Y/D099R/G100L，A098Y/D099R/G100Z/S101Z/G102D，A098Z/S101R，Z098.01N，Z098.01R，Z098.01R/G100R，D099C/K136N，D099C/S101D，D099C/S101R，D099C/S101Z，D099C/Z099.01S，D099C/Z100.01G，D099F/S101Z，D099G/G100C，D099G/G100D/S101R，D099G/G100H，D099G/G100R/S101V，D099G/G100S/S101R，D099G/S101G，D099G/S101H，D099G/S101V，D099G/S101Z，D099G/S101Z/N269D，D099G/Z099.01N，D099G/Z099.01R/G100S，D099G/Z099.01S，D099G/Z100.01G，D099G/Z100.01G/S183N/Q275K，D099H/S101Z，D099H/S101Z/V150I，D099H/Z100.01G/A273V，D099H/Z100.01G/Z100.02G，D099I/Z099.01S，D099L/N118D，D099L/S101G，D099L/S101V，D099L/Z100.01G，D099N/A116T，D099N/G100R/S101V，D099N/S101G，D099N/S101H，D099N/S101Z，D099N/Z099.01S，D099N/Z100.01G，D099R/S101D，D099R/S101G，D099R/S101V，D099R/S101Z，D099R/S101Z/A133T，D099R/Z099.01N，D099R/Z099.01S，D099R/Z100.01G，D099R/Z100.01N，D099S/S101G，D099S/S101I，D099S/S101Z，D099S/Z099.01H，D099S/Z099.01L，D099S/Z099.01V，D099S/Z100.01G，D099S/Z100.01G/Z100.02G，D099V/S101D，D099V/S101V，D099V/S101Z，Z099.01H/S101L，Z099.01H/Z099.02R，Z099.01N/S101R，Z099.01S，Z099.01S/S101V，Z099.01S/Z099.02G/A228T，G100D/S101G，G100D/S101R，G100H/G102V，G100H/S101G，G100H/S101R，G100L/S101G，G100L/S101R，G100N/S101D，G100N/S101L，G100N/S101R，G100R/LL267R/I268V，G100R/S101G，G100R/S101G/G160S/S182N，G100R/S101G/Q103Z/Y104Z/S161N，G100R/S101G/S163N，G100R/S101N，G100R/S101R，G100R/S101R/Q103Z/V270I，G100R/S101Z/G102C/Q103D，G100S/S101C，G100S/S101G，G100S/S101R，G100S/S101R，G100S/S101V，G100S/S101V/A231T，Z100.01G，Z100.01L，Z100.01N，Z100.01R，Z100.01R/S101G，S101C/Q103I，S101C/Q103S，S101D/Q103C，S101D/Q103D，S101D/Q103F，S101D/Q103H，S101D/Q103H，S101D/Q103R，S101D/Q103R/H238Y，S101D/Q103S，S101D/Q103V，S101F/Q103G，S101G/A151V，S101G/A179V，S101G/G102S，S101G/Q103G，S101G/Q103L，S101G/Q103N，S101G/Q103R，S101G/Q103R/A179T，S101G/Q103R/G157D，S101G/Q103S，S101G/Q103S/L233S，S101G/Q103S/L233S，S101G/Q245H，S101G/S249N，S101G/V139I，S101G/Z101.01R，S101G/Z101.01R/A200T，S101G/Z102.01G，S101H/G102S，S101H/Q103D，S101H/Q103G，S101H/Q103G/E251Q，S101H/Q103H，S101H/Q103I，S101H/Q103R，S101H/Q103R/A274Z/Q275Z，S101H/Q103S，S101H/Q103V，S101I/Q103R，S101L/G102S/Q103R，S101L/Q103D，S101L/Q103G，S101L/Q103R，S101L/Q103R/A138T，S101L/Q103R/V121I/K213E，S101L/Q103S，S101L/Q103V，S101L/Z101.01R，S101N/Q103R，S101N/Z102.01G，S101R/A151V，S101R/A273V，S101R/E156G，S101R/G102L/Q103G，S101R/G102S，S101R/G102S/Q103R，S101R/G102S/Q103V，S101R/G131D，S101R/Q103C，S101R/Q103D，S101R/Q103D/S248R，S101R/Q103G，S101R/Q103G/A116T，S101R/Q103G/V147I/A153V，S101R/Q103G，S101R/Q103L，S101R/Q103N，S101R/Q103R，S101R/Q103R/S161N，S101R/Q103R/V148I，S101R/Q103S，S101R/Q103S/L126F，S101R/Q103V，S101V/Q103G，S101V/Q103H，S101V/Q103N/Z275.01K，S101V/Q103R，S101V/Q103V，S101Y/G102S/Q103R，S101Y/Q103L，S101Y/Q103S，S101Y/Q103V，S101Z/G102I/Q103H/Y104G/L135F，S101Z/G102V/Q103R/Y104L，S101Z/Q103D，S101Z/Q103F，S101Z/Q103L，S101Z/Q103R，G102C/Q103L/Y104S，G102R/Q103C/Y104C/V192I，G102R/Q103G/Y104R，G102R/Q103R/Z103.01G，G102Z/Q103S，Z102.01G/Q103R，Z102.01G/Q103R/L267V，Z102.01G/Z102.02R/Z102.03G，Q103H/Y104N/Q275Z，Q103N/E156D，Q103R/I122L/N123S/M124V，Q103R/Y104F，Q103Y/S182N，S105G/P129S/S130H/G131H，W106C/A116D/N117S/N118C，N109S/M119C/D120G/V121L，A116G/N117Z/N118R/M119C，A116H/N117G/N118R，N117C/N118G/M119S，N117C/N118G/M119S/A216V，N117G/N118G/M119C，N117G/N118R/M119C，N117H/N118C/M119C，N117H/N118D/M119S，N117H/N118G/M119C，N117H/N118G/M119L，N117H/N118G/M119S，N117H/N118G/M119V，N117H/N118H/M119V，N117H/N118R/M119C，N117H/N118R/M119S，N117H/N118S/M119C，N117H/N118S/M119S，N117H/N118S/M119V，N117I/N118G/M119V，N117L/N118G/M119V，N117R/N118G/M119C，N117R/N118G/M119I/V270I，N117R/N118G/M119S，N117R/N118H/M119V，N117R/N118R/M119C，N117R/N118R/M119I，N117R/N118R/M119S，N117R/N118R/M119V，N117R/N118S/M119I，N117R/N118S/M119L，N117S/N118G/M119C，N117S/N118G/M119S，N117S/N118H/M119V，N117S/N118R/M119V，N117V/N118G/M119C，N117V/N118H/M119C，N117Y/N118G/M119S，N117Y/N118G/M119V，Z117.01G/N118R/M119C，N118C/M119V/D120S，N118C/Z118.01G/M119C，N118D/M119C/D120L，N118D/M119S/D120R，N118G/M119C/D120G，N118G/M119C/D120L，N118G/M119I/D120R，N118G/M119N，N118G/M119S/D120G，N118G/M119S/D120R，N118G/M119V/D120G，N118G/M119V/D120S/S182N，N118H/M119C/D120R，N118H/M119I/D120G，N118H/M119N/V148I，N118H/M119S/D120G，N118H/M119V/D120G，N118R/M119C，N118R/M119C/D120G，N118R/M119C/D120R，N118R/M119S，N118R/M119S/D120G，N118R/M119S/D120R，N118R/M119S/D120S，N118R/M119V/D120C，N118R/M119V/D120G，N118R/M119V/D120G/A232T，N118S/M119C/D120R，M119C/D120G，M119C/D120G/V121I，M119C/D120G/V121L，M119C/D120H，M119C/D120H/A187V，M119C/D120R，M119H/V121I，M119I/D120C，M119I/D120G，M119I/D120R，M119L/D120G，M119S/D120R，M119S/D120S/V121I，M119S/V121I，M119V/D120C，M119V/D120G，M119V/D120R，M119V/D120S/V198L，D120G/I122C，D120G/I122C/L217S，D120G/I122G，D120G/I122V，D120H/I122L，D120H/V121C/I122V，D120N/V121L/I122V，D120R/A151V，D120R/I122V，D120R/V121I，D120R/V121I/I122C，D120R/V121I/I122C/A228T，D120S/I122C/A133S，V121C/I122C/N123C，V121C/I122G/N123V，V121C/I122S/N123V，V121C/I122V/N123S，V121I/I12C/N123C，V121I/I122C/V150I，V121I/I122G/N123C，V121I/I122S/N123C，V121I/I122S/N123I，V121I/I122V/N123S，V121L/I122S/N123V，V121L/N123C，I122C/N123C，I122C/N123S/M124L，I122D/N123V/M124L，I122G/N123C/M124L，I122G/N123G/Z123.01C，I122H/N123V/M124LI122S/N123S，I122V/N123G/M124C/A138V，I122V/N123S/M124I，I122V/S183G，N123C/M124S，N123C/M124V，N123S/M124L，N123S/M124S，M124C/L126S，M124C/L126S/T244I，M124C/L126V，M124I/L126H，L126F/P129Z，L126F/P129Z/S182N，L126I/P129Z，L126R/G127R，Z126.01S，G127C/G128S/P129R，G127C/P129G，G127C/P129G/A200T，G127C/P129R，G127C/P129R/A153T，G127D/P129G，G127D/P129S，G127H/P129G，G127H/P129R，G127L/P129R，G127N/P129C，G127N/P129G，G127N/P129G/A133T，G127N/P129G/A151V/K213N，G127N/P129R，G127N/P129S/A273V，G127N/P129V，G127R/A230V，G127R/P129D，G127R/P129D/L217S，G127R/P129G，G127R/P129R，G127R/P129S，G127S/P129D，G127S/P129F，G127S/P129G，G127S/P129G/G166D，G127S/P129G/I175T，G127S/P129R，G127S/P129S，G127S/P129V，G127V/P129G，G127V/P129R，Z127.01G/Z127.02H/P129D，Z127.01G/Z127.02H/P129G，Z127.01G/Z127.02H/P129R，Z127.01H/G128C/P129C/A230V，Z127.01H/P129G，Z127.01H/P129G/V148I，Z127.01H/P129R，Z127.01N/G128C/P129V，Z127.01N/G128S/P129S，Z127.01N/G128S/P129V，Z127.01N/P129R，Z127.01R/G128S/P129S，Z127.01R/P129G，Z127.01S/P129D，Z127.01S/P129G，Z127.01S/P129R，G128D/P129G，G128D/P129R/Z129.01R，G128D/P129S/G154S，G128H/P129C，G128H/P129H/Z129.01G，G128H/P129H/Z129.01R，G128H/P129R，G128H/P129R/Z129.01G，G128H/P129R/Z129.01R，G128H/P129S/Z129.01G，G128H/P129S/Z129.01R，G128H/P129S/Z129.01R/S248N，G128H/P129Y，G128H/Z128.01R/P129D，G128H/Z128.01R/P129S，G128H/Z128.01R/P129S/A144T，G128H/Z129.01R，128N/P129R/Z129.01G，G128N/P129R/Z129.01R，G128N/P129S/Z129.01D，G128N/Z128.01R/P129S，G128R/P129G，G128R/P129H/Z129.01G，G128R/P129L/Z130.01S，G128R/P129R，G128R/P129R/Z129.01G，G128R/P129R/Z129.01G/A216V，G128R/P129S，G128R/P129S/Z129.01G，128R/P129Z/S130R/S132G，G128R/Z128.01R/P129D，G128R/Z128.01R/P129F，G128S/P129C，G128S/P129C/Z129.01R，G128S/P129D，G128S/P129D/S248R，G128S/P129D/T244N，G128S/P129G，G128S/P129G/A187V，G128S/P129H/Z129.01R，G128S/P129R/L209F，G128S/P129R/Z129.01G，G128S/P129S/Z129.01D，G128S/P129S/Z129.01R，G128S/P129V，G128Y/P129R/Z129.01G，Z128.01G，Z128.01G/P129D，Z128.01G/P129R，Z128.01G/P129S/A134T，Z128.01G/Z128.02G，Z128.01R/P129D，Z128.01R/Z128.02G/P129S，Z128.01R/Z128.02H/P129G/G160S，Z128.01R/Z128.02R/P129D，Z128.01Y/Z128.02H/P129R，P129C/S130C/S132Z，P129D/Z129.01G/Z129.02D/G160D，P129D/Z129.01G/Z129.02G，P129G/G131Z，P129G/S130G/Z131.01G，P129G/S130H/S132Z，P129G/S130R/S132Z，P129G/V270I，P129G/Z129.01G，P129G/Z129.01R/Z129.02G，P129H/G131Z，P129H/G131Z/S132C，P129H/G131Z/S132H，P129H/S132Z，P129H/Z130.01D/Z130.02S，P129R/G131Z/S132H，P129R/S130D/S132Z，P129R/S130N/G131Z，P129R/S132T，P129R/S132Z，P129R/Z129.01G，P129R/Z129.01G/Z129.02G，P129R/Z129.01G/Z129.02G/A144T，P129R/Z129.01G/Z129.02G/G146D，P129R/Z129.01G/Z129.02R，P129R/Z129.01G，P129R/Z129.01R/Z129.02G，P129S/G131ZP129S/S130H/S132Z，P129S/Z129.01C，P129S/Z129.01R/Z129.02S，P129S/Z130.01N/Z130.02S，P129V/S132Z，P129Z，P129Z/G131R/S132C，P129Z/S130G，P129Z/S130G/G131H/S132H，P129Z/S130G/G131V，P129Z/S130H，P129Z/S130H/S132G，P129Z/S130H/S132N，P129Z/S130L，P129Z/S130R，P129Z/S130R/G131C，P129Z/S130R/G131C，P129Z/S130R/G131D/S132C，P129Z/S130R/G131H/A144E，P129Z/S130R/G131H/S132D，P129Z/S130R/G131R/S132D，P129Z/S130R/L267M，P129Z/S130R/S132C，P129Z/S130R/S132G，P129Z/S130V/S132G，P129Z/S130Z/G131N/S132G，S130D/G131C/S132D，S130D/G131R，S130D/G131R/S132D，S130D/G131Z，S130D/G131Z/S132H，S130F/G131N/S132V，S130F/G131S/S132V，S130F/S132V，S130G/G131D/S132C，S130G/G131H/S132D，S130G/G131S/S132I，S130G/S132G，S130H/G131C/S132G，S130H/G131H/S132R，S130H/G131R/S132C，S130H/G131S，S130H/G131S/S132C，S130H/S132Z，S130I/G131S/S132H，S130I/S132G，S130N/G131S/S132C，S130R/G131R/S132C，S130R/G131S/S132C/S161N，S130R/S132G，S130R/S132G/S183N，S130R/S132Z，S130V/G131D/S132I，S130V/G131H/S132C，S130V/S132N，S130V/S132Z，S130Y/G131H/S132C，S130Y/S132G，S130Z/G131H/S132H，G131H/S132L/W241R，G131N/S132C，G131S/S132G，G131Y/S132G和A142V/G211V，其中所述位置对应于BPN’枯草蛋白酶SEQ ID NO：2的位置。